Zymomonas mobilis Mutants with an Increased Rate of Alcohol Production
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چکیده
منابع مشابه
Production of Acetaldehyde by Zymomonas mobilis.
Mutants of Zymomonas mobilis were selected for decreased alcohol dehydrogenase activity by using consecutively higher concentrations of allyl alcohol. A mutant selected by using 100 mM allyl alcohol produced acetaldehyde at a level of 4.08 g/liter when the organism was grown in aerated batch cultures on a medium containing 4.0% (wt/wt) glucose. On the basis of the amount of glucose utilized, th...
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Respiratory chain analysis of high ethanol producing Zymomonas mobilis mutants. 1 2 Takeshi Hayashi* Tsuyoshi Kato, and Kensuke Furukawa 3 4 Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu 5 University, Beppu, Oita 874-8501, Japan 6 Food Science and Nutrition, Graduate School of Food Science and Nutrition, Beppu 7 University, Beppu, Oita 874-8501, Japan 8 9 *Co...
متن کاملDifferential Expression of Zymomonas mobilis Sucrase Genes (sacB and sacC) in Escherichia coli and Sucrase Mutants of Zymomonas mobilis
The sacB and sacC genes encoding levansucrase and extracellular sucrase respectively were independently subcloned in pBluescript (high copy number) and in Z. mobilis-E. coli shuttle vector, pZA22 (low copy number). The expression of these genes were compared under identical background of E. coli and Z. mobilis host. The level of sacB gene expression in E. coli was almost ten fold less than the ...
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It has been reported in literature that Z. mobilis has two types of alcohol dehydrogenase gene (ADH), one is Zinc inducible, and another is Iron (Ferrous) inducible. Therefore, it was attempted to increase the alcohol production by inducing the alcohol dehydrogenase activity by addition of Zinc ions. It was standardized that 10 mM Zinc sulphate addition to alcohol producing broth resulted in ma...
متن کاملAlcohol Production from Cassava Starch by Co-immobilized Zymomonas mobilis and Immobilized Glucoamylase
Simultaneous saccharification and fermentation of dextrin£zed cassava starch to glucose and alcohol, respectively, were carried out by co-immobilized Zymomonas mobilis and immobilz'zed glucoamylase (IG). Calcium alginate-entrapped cells and IG (4 : 1 ratio) gave an alcohol productivity of 0.30 glgww cellslh in a batchfermentation process. For continuous fermentation, 54.3 gil alcohol was produc...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1987
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.53.7.1425-1432.1987